Understanding and interpreting serum protein electrophoresis. Capillarys 3 tera from sebia is a multiparametric capillary electrophoresis instrument which can analyze serum proteins and perform their immunotyping. Streamlined signout of capillary protein electrophoresis. Capillary zone electrophoresis sebia tests gtr ncbi.
The sebia capillarys 3 tera is an automated analyzer based on capillary zone electrophoresis and uv detection for the quantitative analysis of hba 1c, proteins serum and urine, immunotyping serum and urine, and carbohydrate deficient transferrin. Alpha thalassemia results from large deletions in the alpha genes. Evaluation of capillary electrophoresis assay for cdt on sebia s capillarys system. Hemoglobin fractions were determined by capillary electrophoresis. Electrophoresis was performed using an automated system capillarys 2. The minicap performs all procedural sequences automatically to obtain a protein profile for qualitative analysis. Screenshot of the interpretation and validation software, phoresis, allowing the visualization of the fractions migration position red circle hb hope hb d hb s hb e hb c min 2 207 2 227 252 max 4 208 214 228 253 mean 2,8 207,1 2,5 227,7 252,2. Sebia, paris, france according to the procedures described by the manufacturer. The hb aic capillary controls are designed for the quality control of human glycated hemoglobin a ic quantification with sebia capillary electrophoresis procedures using the minicap hba ic kit with the mini cap flexpiercing automated instrument the hb a ic capillary controls are designed for professional use only. They feature whole blood analysis, with no preanalytical preparation.
Capillary zone electrophoresis cze at alkaline ph is one of the techniques used for hemoglobinopathy screening. Sebia electrophoresis educational material cd presentations. Hemoglobin electrophoresis is a wellestablished technique routinely used in clinical laboratories to screen samples for hemoglobinopathies hemoglobin variants and thalassemias the capillarys hemoglobine kit enables, on capillarys instruments, the efficient separation of hemoglobin fractions and detection of a large number of hemoglobin variants and thalassemias patterns. The use of capillary electrophoresis for hemoglobinopathies diagnosis. Sebia electrophoresis company details sebia electrophoresis from the highest quality manual the completely automated capillarys 2 capillary electrophoresis system, sebia continues. The capillarys hemoglobine kit is designed for laboratory use. It is used in conjunction with capi 3 proteine 6 kit, sebia, designed for protein separation into 6 major fractions in alkaline buffer ph 9. The capillarys system, sebia, for capillary electrophoresis has been developed to provide complete automation of this testing with fast separation and good resolution. See the sebia capillarys 2 flexpiercing instruction manual.
The capillarys hemoglobine assay is based on the principle of capillary electrophoresis in free solution. The sebia capillarys capillary zone electrophoresis ce and the primus resolution highpressure liquid chromatography hplc were used to prospecti we use cookies to enhance your experience on our website. Capillary electrophoresis system all medical device. Sebia capillarys 2 flex piercing and high performance liquid. Sebia s capillarys 2 and minicap instruments utilize innovative capillary electrophoresis ce technology, an alternative to traditional agarose gel electrophoresis. Physician pathologist interpretation of electrophoretic. The sebia capillarys 2 neonat fast system is now available as part of perkinelmer s total newborn screening solution. Endeavors in capillary electrophoresis ce began as early as the late 1800s. For the daily sample probe and capillaries cleaning in automated system minicap, sebia, for capillary electrophoresis. Hemoglobin fractions are separated in silica capillaries, by their electrophoretic mobility and electroosmotic flow at a high voltage in an alkaline buffer.
For rinsing capillaries in automated instrument capillarys 2 flexpiercing, sebia, for capillary electrophoresis. The capillarys 2 flexpiercing instrument uses the principle of capillary electrophoresis in free solution. In addition to capillary electrophoresis, the provide a comprehensive range of agarose gel electrophoresis instruments and tests. In this report, we evaluate automated capillary zone electrophoresis by capillarys sebia, france. Apply in a microtube 1 ml of capillarys minicap cdt wash solution. Charged molecules are separated by their electrophoretic mobility at a specific ph in an alkaline buffer. This device has been validated for use with the sebia capillarys system capillary electrophoresis. Within this presentation, discover the science and principles behind ce technology and eof. Capillary electrophoresis is a physical method of analysis based on the migration, inside a capillary, of charged analytes dissolved in an electrolyte solution under the influence of a directcurrent electric field. Methods and materials sebia sebia, france hemoglobin electrophoresisis at ph 8. Evaluation of the sebia minicap flex piercing capillary electrophoresis for. Capillary electrophoresis is a separation method performed by applying a high voltage along a capillary. They exclude delivery charges and customs duties and do not include.
Capillary electrophoresis ce has become a technique of choice for the. Pdf the sebia capillarys capillary zone electrophoresis ce and the primus resolution highpressure liquid chromatography hplc were. Experiments began with the use of glass u tubes and trials of both gel and free solutions. The hydragel 4 if kit is designed for the detection of monoclonal proteins in human serum and urine by immunofixation electrophoresis. Hbny discovered through hba1c analysis using capillary electrophoresis capillarys 2. Both capillary electrophoresis assays and agarose gel, and their dedicated automation, are designed to be integrated into the same routine workflow. The capillarys hba 1c kit sebia, lisses, france is designed for the separation and quantification of the hba 1c fraction of hemoglobin in human blood by means of capillary electrophoresis in an alkaline buffer with the capillarys 2 flex piercing instrument sebia. Aug 08, 2016 the capillarys 3 tera instrument uses the principle of capillary electrophoresis in free solution which is the most common form of capillary electrophoresis.
Abstract capillary zone electrophoresis cze of serum proteins is increasingly gaining impact in clinical laboratories. A large band of hb a and a small band of hb h are seen. Karen anderson 1705 corporate drive, suite 400 trade. Technology serum and urine capillary electrophoresis. It is also able to manage whole blood on capped tube at a high throughput up to 70 sampleshour, allowing the measurement of hb a1c.
In addition to the three capillary electrophoresis systems, we are considering the implementation of an agarose gel chain to perform urine electrophoresis with the hydrasys 2 scan instrument and the assist sampler that provides traceability of the results. Separation of serum proteins by capillary electrophoresis. Separation occurs according to the electrolyte ph and electroosmotic flow. Capillarys hb a1c using the capillarys 2 flexpiercing instrument. Text books solely focused on the discipline of electrophoresis testing can be difficult to find. Comparison between capillary zone electrophoresis and capillary. It is recommended to filter distilled or deionized water with 0. The kits are used in conjunction with the semiautomated hydrasys electrophoresis apparatus. Jul 14, 2020 sebia has adapted highresolution capillary electrophoresis technology on its capillarys 3 tera instrument for an accurate hba1c measurement at a very high speed. Recognize the most important expected test results in hemoglobinopathies and. Evaluation of the sebia capillarys 2 flex piercing for the. Since the introduction of capillary electrophoresis, which has a better resolution than gel electrophoresis, and the contribution of the free light chain assay which makes it possible to detect the free light chain components, an immunotyping analysis is carried out in 10 % of explorations. Evaluation of the sebia capillarys 3 tera and the biorad. Performance of the sebia capillarys 2 for detection and.
Karen anderson 1705 corporate drive, suite 400 tradedevice. Evaluation of the sebia capillarys 3 tera and the biorad d. Comparison of sebia capillarys capillary electrophoresis. Hemoglobin fractions are directly detected at the specific absorbance of 415 nm. By continuing to use our website, you are agreeing to our use of cookies. Sebia is pleased to offer text books specifically related to serum and urine protein electrophoresis and immunofixation testing, as well as the diagnosis of hemoglobinopathies with capillary electrophoresis technology. View pdf catalogues and other online documentation prices are pretax. Jan 01, 2018 capillary electrophoresis estimates monoclonal peaks more accurately, because there are no varying dye binding affinities of proteins as seen with agarose gel electrophoresis. Identification of haemoglobin new york by haemoglobin a.
For capillaries rinsing in automated system capillarys, sebia, for capillary electrophoresis. Sebia capillarys zone electrophoresis system for monoclonal paraprotein analysis. Withinrun and betweenrun imprecision for the five electrophoretic fractions was electrophoresis has been the method of choice for the identification and quantification of different hemoglobins. Hb a1c venous blood hb a1c capillary blood serum protein electrophoresis spe urine protein electrophoresis upe serum immunotyping urine immunotyping cdtcdtifcc. The quantification of hba 1c with capillary electrophoresis capillarys 2 flex piercing analyzer, sebia, lisses, france was not influenced by the presence of carbamylated hemoglobin a or labile a 1c b in the range tested up to 5.
Sep 07, 2020 the sebia systems use the principle of capillary electrophoresis in free solution. For optimal operation, add 35 ldl of clean protect sebia, pn 2059, 1 vial of 5 ml. Highresolution hba1c separation and hemoglobinopathy. Comparison of sebia capillarys capillary electrophoresis with the. Expression of hemoglobin variant migration by capillary. Hb h is an unstable hemoglobin which causes a hemolytic anemia. It is used in conjunction with the minicap proteine 6 kit, sebia, designed for protein separation into 6 major fractions in alkaline buffer ph 9. To prevent microbial proliferation, change the water every day. Sebia enters into license agreement with erasmus mc and.
The better differentiation of the beta region into beta1 and beta2globulin fractions also improves their detection 7, 8. Pdf automated serum protein electrophoresis by capillarys. The capillarys 3 tera instrument performs all procedural sequences automatically to obtain a protein profile for qualitative analysis. Capillary electrophoresis of serum proteins across the. Immunotyping performed using capillary electrophoresis on sebia capillarys 2 and capillarys 2 flex piercing instruments and on sebia minicap instrument, immunofixation methods. Sebia electrophoresis company details medical sebia has been 100% focused on the science of electrophoresis. From whole blood sampling in primary capped tubes to the final result, the capillarys 3 tera instrument performs all sequences automatically. The history and findings are typical of hb h disease, usually due to the inheritance of a total of three deleted alpha chain genes. The next generation of hba1c testing, based on the principle of capillary separation. See the sebia capillarys 2 flexpiercing instruction man. In discrepant cases samples were run on sebia capillary zone electrophoresis cze for confirmation of results 39 out of 107 cases with hbe, hbd iran, hb lepore and hbq. Analysis of serum proteins by electrophoresis is routinely used to screen. Capillary electrophoresis is a physical method of analysis based on the migration, inside a capillary, of analytes dissolved in an electrolyte solution under the influence of a directcurrent electric field.
Now, immunosubtraction by automated technology is used in many laboratories that employ capillary zone electrophoresis. Carbohydrate deficient transferrin a guide to evaluation and interpretation of electrophoresis run on sebia capillarys 2 april 2016 conference. The sebia capillarys 3 tera instrument includes 12 silica capillaries functioning in parallel. Method comparison and bias estimation using patient samples 2nd edition. Pdf comparison of sebia capillarys capillary electrophoresis. Together perkinelmer and sebia can change your neonatal hemoglobin screening for the better. As all sebia instruments have networking capabilities, we. The capillarys immunotyping pn 2100 kit is designed for optimal performance on the sebia capillarys system pn 1220, an automated capillary electrophoresis system k022227 cleared on july, 2002. The sebia capillarys 2 records hb migration on the xaxis from 0 to 300. From the highest quality manual electrophoresis the completely automated capillarys 2.
Comparison of sebia capillarys capillary electrophoresis with. Intra and inter laboratory precision, reference interval and cutoff schellenberg f, wielders j p. It is recommended to filter distilled or deionized water on 0. Capillary hemoglobin electrophoresis of healthy and. Sebia is pleased to offer text books specifically related to serum and urine protein electrophoresis and immunofixation testing, as well as the diagnosis of hemoglobinopathies with capillary electrophoresis. The electrophoresis system uses the principle of capillarity in free solution to allow multiple simultaneous analyses.
Pdf multicenter validation of fully automated capillary. Hematopathology hb variant migration by ce relative to hba2. In this study, an evaluation of the performance of a lower throughput cze instrument, the sebia minicap flex piercing system, for this purpose is reported for the first time. The migration velocity of the analyte under an electric field of intensity e is determined by the electrophoretic mobility of the. Capillary electrophoresis is a separation technique in which charged species are separated, based on charge and size, by their different rates of migration i. Automated multicapillary electrophoresis for analysis of. Separation also occurs according to the electrolyte. Result interpretation software powerful phoresis core software makes your patient management easy multiparametric instruments with large range of tests. We compared the new fully automated capillary electrophoresis system from sebia, capillarys.
The sebia capillarys capillary zone electrophoresis ce and the primus resolution highpressure liquid chromatography hplc were used to prospectively evaluate 297 samples for hemoglobinopathies. Hemoglobin electrophoresis is a well established technique routinely used in clinical. Methods haemoglobin separation haemoglobin separation was performed with a. Using data innovations instrument manager, an interface was established with the lis cerner that allowed for bidirectional transmission of numeric data. Laboratory diagnosis of hemoglobinopathies and thalassemia. These samples were evaluated in three other common hba 1c methods.
Our clinical laboratory implemented capillary electrophoresis using a sebia capillarys2 norcross, ga, usa instrument for serum and urine protein electrophoresis. Capillary zone electrophoresis sebia gtr test id help each test is a specific, orderable test from a particular laboratory, and is assigned a unique gtr accession number. Capillary electrophoresis for the screening and diagnosis of. Protein electrophoresis is a well established technique routinely used in clinical laboratories for screening urine and serum samples for protein abnormalities 3, 5, 7, 9, 10, 18. Eventually, sequencing analysis of genes confirmed a missense mutation cccgcc at codon 114 in.
Evaluation of the sebia minicap flex piercing capillary. Capillarys 2 flex piercing detected a rare case of hb broomhill. Riou j, godart c, wajcman h, bardakdjianmichau j genetic biochemistry department, u955 inserm unit, henri mondor hospital, creteil, france. The electrophoresis apparatus capillarys 2 from sebia figure 2. This mode of capillary electrophoresis is appropriate for the analysis of small mw capillary zone electrophoresis, separation of molecules having only minute differences in their chargetomass ratio can be effected. Both the hplc and cze methods have the advantage of showing the presence of hemoglobin variants, which is mainly of importance for the interpretation of hba 1c results if there is a high frequency of hemoglobin variants in the background. Withinrun and betweenrun imprecision for the five electrophoretic fractions was oct 16, 2014 introduction. Hemoglobin electrophoresis on cellulose acetate at ph 8. Capillarys 3 tera instrument, sebia, for capillary electrophoresis. Figure 1 hb e analysis of sebia minicap and helena v8 for patient 3.
More recently, capillary zone electrophoresis cze has been introduced for the hba 1c determination. Manufacturers recommended normal ranges for healthy adults were as follows. Oct 02, 2018 sebia are world leaders in electrophoresis testing. The hemoglobins are reported in % units along with an electrophoresis scan. Physician pathologist interpretation of electrophoretic assays. With this technique, charged molecules are separated by their electrophoretic mobility in an alkaline buffer with a specific ph.
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